Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RCCR Polyclonal antibody

Uniprot No.

Q8LDU4

Target Names

RCCR

Alternative Names

RCCR antibody; ACD2 antibody; At4g37000 antibody; C7A10_360 antibody; Red chlorophyll catabolite reductase antibody; chloroplastic antibody; AtRCCR antibody; RCC reductase antibody; EC 1.3.7.12 antibody; Accelerated cell death protein 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RCCR protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalyzes the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC). Works in a two-step reaction with pheophorbide a oxygenase (PaO) by reducing the C20/C1 double bond of the intermediate, RCC. Belongs to the chlorophyll catabolic enzymes (CCEs).

Gene References into Functions

ACD2 localizes dynamically during infection to protect cells from pro-death mobile substrate molecules, some of which may originate in chloroplasts, but have major effects on mitochondria. PMID: 21988537
Two conserved acidic residues, Glu154 and Asp291, of chlorophyll catabolite reductase sandwich the C20/C1 double bond of red chlorophyll catabolite, suggesting that these two residues are involved in site-specific reduction. PMID: 20727901
Used to elicit the role of mitochondria in programmed cell death as evaluated by flow cytometry. PMID: 15500474
The roles of ACD2 and PAO1 enzymes in chlorophyll breakdown during leaf sensescence in A. thaliana are reported. PMID: 16113212
The Arabidopsis thaliana chloroplast protein ACCELERATED CELL DEATH2 (ACD2) modulates the amount of programmed cell death (PCD) triggered by Pseudomonas syringae and protoporphyrin IX (PPIX) treatment. PMID: 16387834
RCCR (At4g37000), together with pheophorbide a oxygenase, is required for the detoxification of chlorophyll catabolites. PMID: 17237353
crystal structure of RCCR at 2.4 A resolution shows RCCR forms a homodimer, in which each subunit folds in an alpha/beta/alpha sandwich. PMID: 19374909

Hide All

Subcellular Location

Plastid, chloroplast stroma. Plastid, chloroplast thylakoid membrane. Note=And a low amount in mitochondria of 7-day-old seedlings.

Tissue Specificity

Expressed in all tissues tested, including roots.

Database Links

KEGG: ath:AT4G37000

STRING: 3702.AT4G37000.1

UniGene: At.4644

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RCCR Antibody

Product Details

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Target Background