Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RCF3 Polyclonal antibody

Uniprot No.

Q8W4B1

Target Names

RCF3

Alternative Names

RCF3 antibody; ESR1 antibody; HOS5 antibody; SHI1 antibody; At5g53060 antibody; MNB8.12 antibody; RNA-binding KH domain-containing protein RCF3 antibody; Protein ENHANCED STRESS RESPONSE 1 antibody; Protein HIGH OSMOTIC STRESS GENE EXPRESSION 5 antibody; Protein REGULATOR OF CBF GENE EXPRESSION 3 antibody; Protein SHINY 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RCF3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts as negative regulator of osmotic stress-induced gene expression. Involved in the regulation of thermotolerance responses under heat stress. Functions as an upstream regulator of heat stress transcription factor (HSF) genes. Negatively regulates HSFA1A, HSFA1B AND HSFA1D, but positively controls the expression of HSFA1E, HSFA3, HSFA9, HSFB3, and DREB2C. Forms a complex with CPL1 that modulates co-transcriptional processes such as mRNA capping and polyadenylation, and functions to repress stress-inducible gene expression. Regulates pre-mRNA processing under salt stress. Involved in primary miRNA processing and pri-miRNA biogenesis. Binds both intronless and intron-containing pri-miRNAs. Acts as a regulator of biotic stress response gene expression and basal JA-mediated responses involved in defense. Acts as negative regulator of resistance to the fungal pathogen Fusarium oxysporum.

Gene References into Functions

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1. PMID: 26512101
HIGH OSMOTIC STRESS GENE EXPRESSION 5 (HOS5)affects the biogenesis of a subset of miRNA. PMID: 26227967
a KH domain-containing putative RNA-binding protein RCF3 is an important upstream regulator for heat stress-responsive gene expression and thermotolerance in Arabidopsis [RCF3] PMID: 23087326
In fry2 mutants, HOS5 failed to be localized in nuclear speckles but was found mainly in the nucleoplasm. hos5 mutants were impaired in mRNA export and accumulated a significant amount of mRNA in the nuclei, particularly under salt stress conditions. [HOS5] PMID: 24146632
K-homology domain-containing protein named SHINY1 (SHI1) interacts with CPL1 to modulate gene expression. [SHI1] [SHINY1] PMID: 23874224

Hide All

Subcellular Location

Nucleus. Nucleus speckle.

Tissue Specificity

Expressed in roots, cotyledons, leaves, flowers and siliques.

Database Links

KEGG: ath:AT5G53060

STRING: 3702.AT5G53060.1

UniGene: At.27262

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA236768XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

RCF3 Antibody

Product Details

Customer Reviews and Q&A

Target Background