cro Antibody

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Code	CSB-PA365829XA01FSF
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia phage lambda (Bacteriophage lambda) cro Polyclonal antibody

Uniprot No.

P03040

Target Names

cro

Alternative Names

cro antibody; lambdap57 antibody; Regulatory protein cro antibody

Raised in

Rabbit

Species Reactivity

Escherichia phage lambda (Bacteriophage lambda)

Immunogen

Recombinant Escherichia phage lambda (Bacteriophage lambda) cro protein

Immunogen Species

Escherichia phage lambda (Bacteriophage lambda)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Plays an essential role in the switch from lysogenic to lytic state and in fine-tuning the lytic phase activation. Mediates the de-repression of the lytic promoters from repression by cI at an early stage of prophage induction. Turns down the expression of the transcriptional activator cII, a factor that initiates the expression of repressor cI, the major component promoting latency. Additionally, cro is able to directly bind to the promoter region of cI thereby preventing the transcription of cI repressor. These two mechanisms ultimately lead to a strong decrease in the amount of cI that will become unable to maintain the latent phase, allowing the transition to lytic growth. Low level of Cro also stimulates the lytic promoters while cI repressor is still present. Cro-mediated up-regulation of pR and pL ultimately leads to its own increased expression and to a successful switch to lytic growth. The second function assigned to Cro is the fine-tuning of the lytic phase activation. Indeed, cro initially diminishes the strength of activation by turning down the expression of lytic genes as well as its own expression.

Gene References into Functions

Cro-DNA binding may not entail a large conformational change relative to the dominant dimer forms in solution. PMID: 22098751

Database Links

KEGG: vg:2703467

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cro Antibody

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