Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RDH54 Polyclonal antibody

Uniprot No.

P38086

Target Names

RDH54

Alternative Names

RDH54 antibody; TID1 antibody; YBR073W antibody; YBR0715 antibody; DNA repair and recombination protein RDH54 antibody; RAD homolog 54 antibody; Recombination factor TID1 antibody; Two hybrid interaction with DMC1 protein 1) [Includes: DNA topoisomerase antibody; EC 5.99.1.-); Putative helicase antibody; EC 3.6.4.12)] antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RDH54 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in the recombinational repair of double-strand breaks (DSB) in DNA during mitosis and meiosis. Has DNA dependent ATPase activity. Promotes D-loop (displacement loop) formation with RAD51 recombinase. Modifies the topology of double-stranded DNA during the D-loop reaction to facilitate the invasion of the homologous duplex molecule by the initiating single-stranded DNA substrate. Required for adaptation from G2/M checkpoint arrest induced by a double strand break, by participating in monitoring the extent of single-stranded DNA produced by resection of DNA ends. This role is distinct from its roles in recombination. Promotes colocalization of RAD51 and DMC1 during meiotic recombination. Involved in crossover interference.

Gene References into Functions

Rdh54, a chromatin remodeler was identified as the first protein required for template switching that does not affect simple gene conversion. PMID: 25066232
Data indicate that the Rad51-binding domain of Rdh54 resides within its N terminus and is required for Rad51 removal from DNA. PMID: 23798704
Tid1 is a novel target of the DNA damage checkpoint pathway. PMID: 23473644
Rad51-Rad54 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward interhomolog DNA strand exchange. PMID: 22761450
Rad54 and Rdh54 have different in vivo interactions with Rad51. PMID: 21558173
In the absence of the Rad54, Rdh54, and Uls1 translocases, Rad51 accumulates spontaneously on chromatin, blocking cell growth and increasing genomic instability. The translocases dissociate Rad51 from sites of nonspecific dsDNA binding during mitosis. PMID: 20864034
Rdh54p presumably operates via chromatin remodulation at the site of damage rendering DNA accessible to the DNA repair enzymes. PMID: 20297653
Results provide molecular information concerning the role of Rdh54 in homologous recombination and DNA repair, and they also demonstrate the functional significance of Rdh54.Rad51 complex formation. PMID: 16831867
Dmc1 can associate with chromatin in the absence of DNA double-strand breaks, and Tid1 suppresses this association. PMID: 16980587
Results demonstrate translocation of Rdh54 on DNA and extrusion of DNA loops by Rdh54 in an ATP hydrolysis-dependent manner. PMID: 17467735
translocation by Tid1 on DNA can result in disruption of three-stranded DNA structures PMID: 17704061
Rdh54 has a role in chromatin rearrangement during DNA recombination and repair PMID: 18292093

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Subcellular Location

Nucleus.

Protein Families

SNF2/RAD54 helicase family

Database Links

KEGG: sce:YBR073W

STRING: 4932.YBR073W

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RDH54 Antibody

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