RDR2 Antibody

Code CSB-PA529348XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RDR2 Polyclonal antibody
Uniprot No.
Target Names
RDR2
Alternative Names
RDR2 antibody; RDRP2 antibody; SMD1 antibody; At4g11130 antibody; F2P3.11 antibody; RNA-dependent RNA polymerase 2 antibody; AtRDRP2 antibody; EC 2.7.7.48 antibody; Protein SILENCING MOVEMENT DEFICIENT 1 antibody; RNA-directed RNA polymerase 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RDR2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
RNA-dependent direct polymerase involved in the production of small interfering RNAs (siRNAs). Required for the biogenesis of endogenous siRNAs of 24 nucleotide which derive from heterochromatin and DNA repeats such as transposons or endogenous gene tandem repeats, such as repeats present in FWA gene. Involved in transcriptional gene silencing (TGS). Component of the RNA-directed DNA methylation (RdDM) silencing pathway that utilizes siRNAs to guide DNA methyltransferases to asymmetric cytosines. Involved in control of flowering time through RdDM of FWA locus. Required for reception of long-distance mRNA silencing in the shoot. Required for the formation of telomeric siRNAs and the RNA-dependent DNA methylation of asymmetric cytosines in telomeric (5'-CCCTAAA-3') repeats.
Gene References into Functions
  1. JMJ24 binds to RDR2 and is required for the basal level transcription of silenced loci in Arabidopsis. PMID: 26119694
  2. These findings begin to reveal features of Pol IV/RDR2-mediated transcription at the heart of genome stability in plants. PMID: 25414514
  3. Results suggest that the physical and mechanistic coupling of Pol IV and RDR2 results in the channeled synthesis of double-stranded precursors for siRNA biogenesis. PMID: 23142082
  4. The data suggest a mechanism whereby IBM1 regulates a series of protein-coding genes including RDR2 through the RdDM-dependent control of siRNA biogenesis. PMID: 22772985
  5. RDR2 plays a significant role in regulating the expression of not only transposons, but also of genes. PMID: 19936292
  6. RDR2 is required for reception of long-distance mRNA silencing. PMID: 17785412

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Subcellular Location
Nucleus, nucleoplasm. Nucleus, nucleolus.
Protein Families
RdRP family
Database Links

KEGG: ath:AT4G11130

STRING: 3702.AT4G11130.1

UniGene: At.43417

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