RNA-directed RNA polymerase subunit beta Antibody

Code CSB-PA526479XA01ELC
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Product Details

Uniprot No.
Alternative Names
RNA-directed RNA polymerase subunit beta antibody; EC 2.7.7.48 antibody; RNA replicase beta chain antibody
Raised in
Rabbit
Species Reactivity
Enterobacteria phage MX1 (Bacteriophage MX1)
Immunogen
Recombinant Enterobacteria phage MX1 (Bacteriophage MX1) RNA-directed RNA polymerase subunit beta protein
Immunogen Species
Enterobacteria phage MX1 (Bacteriophage MX1)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This is the catalytic subunit of the viral RNA-dependent RNA polymerase complex. This complex is involved in viral RNA replication that produces (+)-stranded genomes via a complementary, (-)-stranded intermediate. Binds RNA cooperatively with the host ribosomal protein S1.
Gene References into Functions
  1. The data suggest that the beta-subunit and protein S1 cooperatively bind the (+)-stranded Qbeta genome during replication initiation. PMID: 26578560
  2. The third mobile OB-fold of ribosomal protein S1, which is spatially anchored near the surface of the Qbeta replicase beta-subunit, primarily recruits the Qbeta RNA toward the beta-subunit, leading to the specific and efficient replication initiation of Qbeta RNA. PMID: 25122749
  3. Thr210Ala and Tyr410His mutation in replicase had little or no effect on replication fidelity. PMID: 24965463
  4. Data indicate that ribosomes enhanced maximum rate of RNA synthesis by Qbeta replicase. PMID: 24269228
  5. EF-Tu in Qbeta replicase modulates RNA elongation processes in a distinct manner from its established function in protein synthesis. PMID: 22245970
  6. Cys533 of the beta Subunit is responsible for replicase Stability. PMID: 20858892
  7. crystal structure of the complex between the beta-subunit and the two host proteins EF-Tu and EF-Ts to 2.5-A resolution PMID: 20534494

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Database Links

KEGG: vg:1261500

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