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| Code | CSB-PA341363XA01SVG |
| Size | Enquire |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Full Product Name | Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REC114 Polyclonal antibody |
| Uniprot No. | P32841 |
| Target Names | REC114 |
| Alternative Names | REC114 antibody; YMR133W antibody; YM9375.02 antibody; Meiotic recombination protein REC114 antibody |
| Raised in | Rabbit |
| Species Reactivity | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Immunogen | Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REC114 protein |
| Immunogen Species | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Conjugate | Non-conjugated |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification Method | Protein A/G |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Form | Liquid |
| Tested Applications | ELISA, WB (ensure identification of antigen) |
| Protocols | ELISA Protocol |
| Troubleshooting and FAQs | Antibody FAQs |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Value-added Deliverables | ① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control); |
| Quality Guarantee | ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive; |
| Lead Time | Made-to-order (14-16 weeks) |
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| Function (From Uniprot) |
Required for the production of double-strand breaks (DSBs) in meiotic recombination initiation; not required for mitosis. Plays a role in activation of the DSB nuclease SPO11 at recombination hotspots. Prevents accumulation of meiotic double strand break intermediates and is needed for the proper timing of the first meiotic division. Component of the MER2-MEI4-REC114 complex which seems to be required for meiotic double-strand break (DSB) formation. |
| Gene References into Functions |
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| Subcellular Location | Nucleus. Chromosome. Note=Localizes to chromosomes. |
| Protein Families | REC114 family |
| Database Links |
KEGG: sce:YMR133W STRING: 4932.YMR133W |
