REB1 Antibody

Code CSB-PA321978XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REB1 Polyclonal antibody
Uniprot No.
Target Names
REB1
Alternative Names
REB1 antibody; GRF2 antibody; YBR049C antibody; YBR0502 antibody; DNA-binding protein REB1 antibody; QBP antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REB1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromatin to a promoter.
Gene References into Functions
  1. Abf1, Reb1, and Tbf1 control ribosome biogenesis gene expression in budding yeast. PMID: 28158860
  2. Heterozygous diploid knockout strains for FHL1, RAP1, REB1, and MCM1 genes showed significantly short lifespan. PMID: 26022127
  3. Data show that the transcriptional activator Reb1p bound to DNA is a roadblock for RNAPII, which pauses and is ubiquitinated, thus triggering termination PMID: 25479637
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YBR049C

STRING: 4932.YBR049C

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