REF6 Antibody

Code CSB-PA871158XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) REF6 Polyclonal antibody
Uniprot No.
Target Names
REF6
Alternative Names
REF6 antibody; JMJ12 antibody; PKDM9A antibody; At3g48430 antibody; T29H11_50 antibody; Lysine-specific demethylase REF6 antibody; EC 1.14.11.- antibody; Jumonji domain-containing protein 12 antibody; Lysine-specific histone demethylase REF6 antibody; Protein RELATIVE OF EARLY FLOWERING 6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana REF6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me. Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay. Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli. Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation. Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression. Interacts with the NF-Y complexe to regulate SOC1. Mediates the recruitment of BRM to its target loci.
Gene References into Functions
  1. BES1 recruits transcriptional regulators ELF6 and REF6 to regulate target gene expression and coordinate brassinosteroids responses with other developmental processes such as control of flowering time. PMID: 18467490
Subcellular Location
Nucleus.
Protein Families
JHDM3 histone demethylase family
Tissue Specificity
Highly expressed in the shoot apical meristem and primary and secondary root tips, and lower expression in cotyledons, leaves and root axis along vascular tissues. Detected in inflorescences, stems and siliques.
Database Links

KEGG: ath:AT3G48430

STRING: 3702.AT3G48430.1

UniGene: At.3169

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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