II Antibody

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Code	CSB-PA322209XA01ECP
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Product Details

Full Product Name

Rabbit anti-Enterobacteria phage I2-2 (Bacteriophage I2-2) II Polyclonal antibody

Uniprot No.

P15419

Target Names

Alternative Names

II antibody; Replication-associated protein G2P antibody; Rep antibody; EC 3.1.21.- antibody; EC 6.5.1.1 antibody; G2P antibody; Gene 2 protein antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage I2-2 (Bacteriophage I2-2)

Immunogen

Recombinant Enterobacteria phage I2-2 (Bacteriophage I2-2) II protein

Immunogen Species

Enterobacteria phage I2-2 (Bacteriophage I2-2)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Isoform G2P plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein G2P is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion.; Isoform G10P protein binds to double-stranded DNA and prevents hydrolysis by nucleases. Additionally, G10P is an inhibitor of DNA replication and may have a role in the transition from semiconservative replicative form DNA replication to single-stranded DNA synthesis in the life cycle.

Protein Families

Inovirus G2P protein family

Database Links

KEGG: vg:1260717

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II Antibody

Product Details

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Target Background