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| Code | CSB-PA356164XA01SVG |
| Size | Enquire |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Full Product Name | Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REP2 Polyclonal antibody |
| Uniprot No. | P03872 |
| Target Names | REP2 |
| Alternative Names | REP2 antibody; R0040C antibody; Partitioning protein REP2 antibody; R2 antibody; Protein Charlie antibody; Trans-acting factor C antibody |
| Raised in | Rabbit |
| Species Reactivity | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Immunogen | Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REP2 protein |
| Immunogen Species | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Conjugate | Non-conjugated |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification Method | Protein A/G |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Form | Liquid |
| Tested Applications | ELISA, WB (ensure identification of antigen) |
| Protocols | ELISA Protocol |
| Troubleshooting and FAQs | Antibody FAQs |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Value-added Deliverables | ① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control); |
| Quality Guarantee | ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive; |
| Lead Time | Made-to-order (14-16 weeks) |
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| Function (From Uniprot) |
Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. |
| Subcellular Location | Nucleus. Note=Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases. |
