A Antibody

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Code	CSB-PA366002XA01FSI
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) A Polyclonal antibody

Uniprot No.

P03631

Target Names

Alternative Names

A antibody; Replication-associated protein A antibody; EC 3.1.21.- antibody; EC 6.5.1.1 antibody; RepA antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174)

Immunogen

Recombinant Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174) A protein

Immunogen Species

Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates in the presence of host Rep and DNA polymerase III at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein A is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion.; The A* protein binds to double-stranded DNA and prevents hydrolysis by nucleases. Additionally, A* is an inhibitor of DNA replication and may have a role in the transition from semiconservative replicative form DNA replication to single-stranded DNA synthesis in the life cycle.

Protein Families

Microviridae Rep protein family

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A Antibody

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