prfA Antibody

Datasheet

Code	CSB-PA015197XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) prfA Polyclonal antibody

Uniprot No.

P0A7I0

Target Names

prfA

Alternative Names

prfA antibody; sueB antibody; uar antibody; b1211 antibody; JW1202 antibody; Peptide chain release factor RF1 antibody; RF-1 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) prfA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.

Gene References into Functions

These results indicate that RF1 undergoes a large conformational change from a closed to an open form upon binding to the ribosome. PMID: 26827724
In a strain lacking RF-1, phosphoserine phosphatase, and where seven UAG codons residing in essential genes were converted to UAA, phosphoserine incorporation into GFP and WNK4 was significantly elevated PMID: 22982858
Release factor one is nonessential in Escherichia coli PMID: 22662873
a conformational change within the RF1.ribosome complex that results in a more tightly bound state PMID: 20873815
RF1 mutations strongly reduce the specific termination activity of RF1 at UAG codons, and lead to hypersensitivity to Kid and RelE toxins. PMID: 19019162
Hypomodification of some tRNAs can suppress the Ts phenotype caused by the RF1 mutant. PMID: 19103926

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Subcellular Location

Cytoplasm.

Protein Families

Prokaryotic/mitochondrial release factor family

Database Links

KEGG: ecj:JW1202

STRING: 316385.ECDH10B_1264

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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prfA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background