prfA Antibody

Code CSB-PA015197XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) prfA Polyclonal antibody
Uniprot No.
Target Names
prfA
Alternative Names
prfA antibody; sueB antibody; uar antibody; b1211 antibody; JW1202 antibody; Peptide chain release factor RF1 antibody; RF-1 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) prfA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.
Gene References into Functions
  1. These results indicate that RF1 undergoes a large conformational change from a closed to an open form upon binding to the ribosome. PMID: 26827724
  2. In a strain lacking RF-1, phosphoserine phosphatase, and where seven UAG codons residing in essential genes were converted to UAA, phosphoserine incorporation into GFP and WNK4 was significantly elevated PMID: 22982858
  3. Release factor one is nonessential in Escherichia coli PMID: 22662873
  4. a conformational change within the RF1.ribosome complex that results in a more tightly bound state PMID: 20873815
  5. RF1 mutations strongly reduce the specific termination activity of RF1 at UAG codons, and lead to hypersensitivity to Kid and RelE toxins. PMID: 19019162
  6. Hypomodification of some tRNAs can suppress the Ts phenotype caused by the RF1 mutant. PMID: 19103926

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Subcellular Location
Cytoplasm.
Protein Families
Prokaryotic/mitochondrial release factor family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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