prfB Antibody

Code CSB-PA963200XA01TNT
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Product Details

Full Product Name
Rabbit anti-Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) prfB Polyclonal antibody
Uniprot No.
Target Names
prfB
Alternative Names
prfB antibody; TTHA0142 antibody; Peptide chain release factor RF2 antibody
Raised in
Rabbit
Species Reactivity
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Immunogen
Recombinant Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) prfB protein
Immunogen Species
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (Probable). In endogenous ribosomes interacts with P-site tRNA and 23S rRNA. In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center and releases the ribosome. Recruited to stalled E.coli 70S ribosomes by E.coli ArfA, but cannot be functionally accomodated in the peptidyl-transferase center. Note T.thermophilus probably does not encode arfA (Ref.1).
Subcellular Location
Cytoplasm.
Protein Families
Prokaryotic/mitochondrial release factor family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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