rfaC Antibody

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Code	CSB-PA338575XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rfaC Polyclonal antibody

Uniprot No.

P24173

Target Names

rfaC

Alternative Names

rfaC antibody; rfa-2 antibody; waaC antibody; yibC antibody; b3621 antibody; JW3596 antibody; Lipopolysaccharide heptosyltransferase 1 antibody; EC 2.-.-.- antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rfaC protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Heptose transfer to the lipopolysaccharide core. It transfers the innermost heptose to [4'-P](3-deoxy-D-manno-octulosonic acid)2-IVA.

Gene References into Functions

HepI enzyme undergoing a substrate binding-induced transition from an open to closed state prior to catalysis. PMID: 23865375
catalytic efficiency of Heptosyltransferase I with the fully deacylated analogue of Escherichia coli HepI LipidA PMID: 22059588
Results report the structure of WaaC alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution. PMID: 16963083
WaaC had access to the substrate before WaaA transfer was completed. PMID: 19201821

Protein Families

Glycosyltransferase 9 family

Database Links

KEGG: ecj:JW3596

STRING: 316385.ECDH10B_3803

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rfaC Antibody

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