rfaH Antibody

Code CSB-PA245393XA01EGY
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O6:K15:H31 (strain 536 / UPEC) rfaH Polyclonal antibody
Uniprot No.
Target Names
rfaH
Alternative Names
rfaH antibody; ECP_4055 antibody; Transcription antitermination protein RfaH antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Immunogen
Recombinant Escherichia coli O6:K15:H31 (strain 536 / UPEC) rfaH protein
Immunogen Species
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Enhances distal genes transcription elongation in a specialized subset of operons that encode extracytoplasmic components. RfaH is recruited into a multi-component RNA polymerase complex by the ops element, which is a short conserved DNA sequence located downstream of the main promoter of these operons. Once bound, RfaH suppresses pausing and inhibits Rho-dependent and intrinsic termination at a subset of sites. Termination signals are bypassed, which allows complete synthesis of long RNA chains. Also negatively controls expression and surface presentation of AG43 and possibly another AG43-independent factor that mediates cell-cell interactions and biofilm formation,.
Protein Families
RfaH family
Database Links

KEGG: ecp:ECP_4055

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