RFT1 Antibody

Code CSB-PA327798XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RFT1 Polyclonal antibody
Uniprot No.
Target Names
RFT1
Alternative Names
RFT1 antibody; YBL020W antibody; YBL0442 antibody; Oligosaccharide translocation protein RFT1 antibody; Requiring fifty-three protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RFT1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in N-linked oligosaccharide assembly. Required for the translocation of Man(5)GlcNAc(2)-PP-dolichol from the cytoplasmic side to the lumenal side of the endoplasmic reticulum membrane.
Gene References into Functions
  1. Although Rft1 may play a critical role in vivo, depletion of this protein does not impair the transbilayer movement of Man5GlcNAc2-P-P-dolichol in sealed microsomal fractions prepared from disrupted cells. PMID: 19494107
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
RFT1 family
Database Links

KEGG: sce:YBL020W

STRING: 4932.YBL020W

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