RGD1 Antibody

Code CSB-PA336439XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RGD1 Polyclonal antibody
Uniprot No.
Target Names
RGD1
Alternative Names
RGD1 antibody; YBR260C antibody; YBR1728 antibody; RHO GTPase-activating protein RGD1 antibody; RhoGAP antibody; Related GAP domain protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RGD1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
GTPase activating protein of RHO3 and RHO4. Acts in concert with MID2 in cell integrity, which is functionally linked to the PKC pathway. Involved in various stress responses. Required at low pH for activation of the PKC pathway. Important during mating response.
Gene References into Functions
  1. demonstrate that Rgd1p is phosphorylated by the Aurora B like kinase Ipl1 and observe that loss of Ipl1 function leads to a new Rgd1p distribution in a small part of the cell population PMID: 23454383
  2. Rgd1p colocalizes with a subset of the post-golgi marker Sec2p and is transported to the bud tip. PMID: 22447923
  3. The authors propose that Tos2p, a protein involved in polarized growth and most probably associated with the plasma membrane, modulates the action of Rgd1p and Rho3p in Saccharomyces cerevisiae. PMID: 21143383
  4. the first evidences for a specific interaction between a RhoGAP domain (the RhoGAP domain of Rgd1p) and phosphoinositides were provided. PMID: 21215255
  5. The rgd1Delta strain is sensitive to low pH, and Rgd1p activates the PKC pathway in an acidic environment PMID: 16087742
  6. Phosphoinositides both regulate the recruitment and trafficking of Rgd1p to membranes via the F-BAR domain and specifically stimulate GTPase-activating protein activity PMID: 18845541

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Subcellular Location
Cytoplasm. Note=Bud and bud neck. During isotropic bud growth in the G1 and S phases, detected at the bud tip. During cytokinesis, undetectable at the bud tip and localized at the bud neck only.
Database Links

KEGG: sce:YBR260C

STRING: 4932.YBR260C

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