RGLG3 Antibody

Code CSB-PA592043XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RGLG3 Polyclonal antibody
Uniprot No.
Target Names
RGLG3
Alternative Names
RGLG3 antibody; At5g63970 antibody; E3 ubiquitin-protein ligase RGLG3 antibody; EC 2.3.2.27 antibody; RING domain ligase 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RGLG3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Possesses E3 ubiquitin-protein ligase in vitro. Acts as upstream modulator of jasmonate (JA) signaling in response to various stimuli, such as JA-inhibited root growth, JA-inductive gene expression, coronatine-mediated pathogen susceptibility, wound-stimulated expression of JA-responsive genes and wound-induced JA biosynthesis. Controls fumonisin B1 (FB1)-triggered programmed cell death (PCD) by modulating the JA signaling pathway. May mediate salicylic acid (SA) suppression of JA signaling in FB1-induced responses. May mediate the formation of 'Lys-48'-linked multiubiquitin chains. Mediates the polyubiquitination and subsequent proteasomal degradation of the target protein GRXS17.
Gene References into Functions
  1. GRXS17 is an ubiquitination target of RGLG3 and is ubiquitinated and degraded in a proteasome-dependent manner by RGLG3. PMID: 27497447
  2. RGLG3 and RGLG4 have coordinated and positive roles in programmed cell death. PMID: 25788731
  3. RGLG3 and RGLG4 are essential upstream modulators of jasmonate signaling in response to various stimuli. PMID: 22898498
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Widely expressed.
Database Links

KEGG: ath:AT5G63970

STRING: 3702.AT5G63970.1

UniGene: At.28971

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