RGP2 Antibody

Code CSB-PA873263XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RGP2 Polyclonal antibody
Uniprot No.
Target Names
RGP2
Alternative Names
RGP2 antibody; At5g15650 antibody; F14F8_30 antibody; UDP-arabinopyranose mutase 2 antibody; EC 5.4.99.30 antibody; Reversibly glycosylated polypeptide 2 antibody; AtRGP2 antibody; UDP-L-arabinose mutase 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RGP2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in vitro. Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Functions redundantly with RGP2 and is essential for proper cell walls and pollen development. Probably involved in the formation of the pectocellulosic cell wall layer intine. Is probably active as heteromer in vivo.
Gene References into Functions
  1. RGP2 cysteine-257 to tyrosine-257 mutation results in the cell wall arabinose deficient Arabidopsis and affects protein stability. PMID: 27217494
  2. RGP2 is required during microspore development and pollen mitosis, either affecting cell division and/or vacuolar integrity. PMID: 17071651
Subcellular Location
Cytoplasm, cytosol. Golgi apparatus.
Protein Families
RGP family
Tissue Specificity
Predominantly expressed in shoot and root apical meristems. Expressed in epidermal cells of leaves, inflorescence stems and seed coat. Expressed in pollen.
Database Links

KEGG: ath:AT5G15650

STRING: 3702.AT5G15650.1

UniGene: At.24638

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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