loco Antibody

Code CSB-PA896074XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) loco Polyclonal antibody
Uniprot No.
Target Names
loco
Alternative Names
loco antibody; CG5248 antibody; Regulator of G-protein signaling loco antibody; RGS antibody; Locomotion defects protein antibody; Loco antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) loco protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI and GAP activities on G(i) alpha subunit Galphai. Confers GAP activity on G(o) alpha subunit Galphao and G(i) alpha subunit Galphai. Involved in the dorsal-ventral axis formation of the egg. Acts as a G-protein signaling for glial cell differentiation during embryogenesis; Galphai, Galphao and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord. May be essential for nurse cell dumping during oogenesis. Required in neuroblast asymmetrical cell division. Plays a role in stress resistance and life span control.
Gene References into Functions
  1. RGS proteins, such as Loco, with with GTPase-activating protein (GAP) activity affect both stress resistance and longevity in several species. PMID: 21255223
  2. Loco was identified and a functional analysis made. PMID: 15313576
  3. specific isoforms of loco are required for the differentiation of the male gonad and genital disc PMID: 18387173
  4. Rap/Fzr targets Loco for ubiquitination, thereby regulating glial differentiation in the developing nervous system. PMID: 18430931
Subcellular Location
Cytoplasm. Cell membrane. Apical cell membrane. Note=Recruited from the cytosol to the plasma membrane by the G(i) alpha subunit. Colocalizes with moody at the plasma membrane. Localizes as a crescent to the apical cortex in interphase and forms an apical crescent and segregates into the apical daughter cell at telophase. Colocalizes with Galphai and raps/pins at the apical cortex throughout mitosis.
Tissue Specificity
Expressed in surface and longitudinal glial cells, gut and heart (at protein level).
Database Links

KEGG: dme:Dmel_CG5248

STRING: 7227.FBpp0083666

UniGene: Dm.3231

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