Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RGS1 Polyclonal antibody

Uniprot No.

Q8H1F2

Target Names

RGS1

Alternative Names

RGS1 antibody; At3g26090 antibody; MPE11.27 antibody; MPE11.28 antibody; Regulator of G-protein signaling 1 antibody; AtRGS1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RGS1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Glucose-regulated GTPase-accelerating protein (GAP) for the GTP-bound self-activating heterotrimeric G alpha protein GPA1. Cooperates with G beta-gamma dimers to maintain an unactivated but fully functional pool of GPA1. Phosphorylation-dependent endocytosis of RGS1 physically uncouples the two proteins, resulting in signal activation. Free AGB1 is essential, but not sufficient, for RGS1 endocytosis. Modulates cell proliferation, abscisic acid (ABA) and drought stress signal transduction by acting in a hexokinase-independent glucose-signaling pathway. Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K(+) and anion channels. Important for the kinetics of voltage activation of inward K(+) current but not for the current amplitude.

Gene References into Functions

complete contrast to G protein activation in animals, plant leucine-rich repeat receptor-like kinases (LRR RLKs), not GPCRs, provide this discrimination through phosphorylation of AtRGS1 in a ligand-dependent manner. PMID: 27235398
It was shown that endocytosis of RGS1 by ligand-dependent endocytosis physically uncouples the GTPase-accelerating activity of RGS1 from the Galpha protein, permitting sustained activation. PMID: 22940907
Functional reconstitution of an atypical G protein heterotrimer and regulator of G protein signaling protein (RGS1) from Arabidopsis thaliana. PMID: 21325279
RGS1 exerted its effects on plant responsiveness to ABA and drought tolerance largely through changing the expression either of genes responsible for ABA biosynthesis or of stress-responsive genes. PMID: 16714312
This finding indicates that RGS1 is important in the dynamics of voltage-dependent activation of Kin current. PMID: 18541915
AtRGS1 combines a receptor-like seven transmembrane domain with an RGS domain, interacts with the AtGPA1 in a d-glucose-regulated manner, and stimulates AtGPA1 GTPase activity. PMID: 18817773

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.

Tissue Specificity

Expressed in hypocotyls, cotyledons, guard cells and root and shoot apical meristems.

Database Links

KEGG: ath:AT3G26090

STRING: 3702.AT3G26090.1

UniGene: At.37280

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RGS1 Antibody

Product Details

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Target Background