Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EIF4A3 Polyclonal antibody

Uniprot No.

Q94A52

Target Names

EIF4A3

Alternative Names

EIF4A3 antibody; RH2 antibody; At3g19760 antibody; MMB12.25 antibody; MMB12.4 antibody; Eukaryotic initiation factor 4A-III homolog antibody; AteIF4AIII antibody; eIF-4A-III antibody; eIF4A-III antibody; EC 3.6.4.13 antibody; DEAD-box ATP-dependent RNA helicase 2 antibody; AtRH02 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EIF4A3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by MLN51/CASC3, but abolished in presence of the MAGO-Y14 heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGO-Y14 heterodimer increases the RNA-binding affinity of the EJC. Plays a role in abiotic stress adaptation. Can regulate abiotic stress resistance partially via the control of acetoacetyl-CoA thiolase 2 (AC Q8S4Y1) expression.; (Microbial infection) Functions as host RNA helicase that is co-opted by the tombusvirus (TBSV) RNA replication enhancer to greatly enhance tombusvirus replication. Interacts with the viral minus-strand RNA and the replication proteins within the viral replicase.

Gene References into Functions

eIF4AIII-like helicases destabilize dsRNA replication intermediate within the RIII(-) REN that promotes bringing the 5' and 3' terminal (-)RNA sequences in close vicinity via long-range RNA-RNA base pairing. PMID: 24743583
Sequestration of eIF4A-III into nuclear pools with different mobility can reflect the transcriptional and mRNA processing state of the cell. PMID: 19435936

Subcellular Location

Nucleus, nucleoplasm. Nucleus, nucleolus. Nucleus speckle. Nucleus. Cytoplasm.

Protein Families

DEAD box helicase family, DDX48/FAL1 subfamily

Database Links

KEGG: ath:AT3G19760

STRING: 3702.AT3G19760.1

UniGene: At.22577

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EIF4A3 Antibody

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