RHD3 Antibody

Code CSB-PA310906XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RHD3 Polyclonal antibody
Uniprot No.
Target Names
RHD3
Alternative Names
RHD3 antibody; FRA4 antibody; At3g13870 antibody; MCP4.11 antibody; Protein ROOT HAIR DEFECTIVE 3 antibody; EC 3.6.5.- antibody; Protein FRAGILE FIBER 4 antibody; Protein SEY1 homolog 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RHD3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable GTP-binding protein involved in cell wall expansion. Required for appropriate root and root hair cells enlargement. May inhibit vacuole enlargement during root hair cell expansion. Plays a role in cell wall biosynthesis and actin organization. Seems to act independently from auxin and ethylene pathways. May regulate membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
Gene References into Functions
  1. This work contributes to a better understanding of a coordinated action of RHD3 in the fusion of endoplasmic reticulum membranes. PMID: 29138354
  2. RHD3 is required for the formation of a fine endoplasmic reticulum structure in the subapical zone of growing root hairs. PMID: 27231102
  3. phosphorylation-stimulated oligomerization of RHD3 enhances endoplasmic reticulum (ER) membrane fusion to form the ER network. PMID: 26684656
  4. and function. RHD3 was initially identified as a gene involved in root hair development. This study uncovered a new physiological function of RHD3 in nitrogen starvation-induced anthocyanin accumulation and ethylene homeostasis. PMID: 25494721
  5. We analyzed the lipid fraction of rhd3 mutants at qualitative and quantitative levels. We observed an increase of the amount of phospholipids but also of proteins in the mutants, indicating an overall increase of ER membranes. PMID: 25763700
  6. required for the unfolded protein response, an essential endoplasmic reticulum signaling pathway PMID: 24876222
  7. RHD3 proteins mediate endoplasmic reticulum fusion and are essential for plant development. PMID: 23922269
  8. The RHD3 C-terminal region has a functional role in endoplasmic reticulum morphology. PMID: 22082223
  9. The expression of mutant forms of RHD3 alters the cellular distribution and motility of Golgi stacks but does not affect endoplasmic reticulum membrane export PMID: 21652628
  10. RHD3 may control epidermal CFR, root skewing, and waving on hard-agar surfaces by regulating the traffic of wall- or plasma membrane-associated determinants of anisotropic cell expansion. PMID: 15908600

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3-type GTPase family, RHD3 subfamily
Tissue Specificity
Highly expressed in fiber and xylem cells. Expressed in roots, cotyledons, seedling hypocotyls, stems, pedicel, stigmatic papillae cells and anthers.
Database Links

KEGG: ath:AT3G13870

STRING: 3702.AT3G13870.1

UniGene: At.23248

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