RHM2 Antibody

Code CSB-PA867982XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RHM2 Polyclonal antibody
Uniprot No.
Target Names
RHM2
Alternative Names
RHM2 antibody; MUM4 antibody; At1g53500 antibody; F22G10.13 antibody; T3F20.18 antibody; Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2 antibody; NDP-rhamnose synthase antibody; Protein MUCILAGE-MODIFIED 4 antibody; Protein RHAMNOSE BIOSYNTHESIS 2 antibody; Rhamnose biosynthetic enzyme 2 antibody; AtRHM2 antibody; UDP-L-rhamnose synthase MUM4) [Includes: UDP-glucose 4,6-dehydratase antibody; EC 4.2.1.76); UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose 4-keto-reductase antibody; EC 1.1.1.- antibody; EC 5.1.3.-)] antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RHM2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Trifunctional enzyme involved in UDP-beta-L-rhamnose biosynthesis, a precursor of the primary cell wall components rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II). Catalyzes the dehydration of UDP-glucose to form UDP-4-dehydro-6-deoxy-D-glucose followed by the epimerization of the C3' and C5' positions of UDP-4-dehydro-6-deoxy-D-glucose to form UDP-4-keto-beta-L-rhamnose and the reduction of UDP-4-keto-beta-L-rhamnose to yield UDP-beta-L-rhamnose. Required for the normal seed coat epidermal development.
Gene References into Functions
  1. Arabidopsis and Camelina sativa, and is regulated by the same transcription factor cascade as endogenous MUM4. PMID: 28730525
  2. Transcription factor GL2 and RHM2 are critical for the biosynthesis of mucilage pectin. PMID: 22735692
  3. A target of GL2 in the seed coat is the gene MUM4 and GL2 regulates seed oil production at least partly through its influence on MUM4 expression in the seed coat. PMID: 21883555
Protein Families
NAD(P)-dependent epimerase/dehydratase family, dTDP-glucose dehydratase subfamily; DTDP-4-dehydrorhamnose reductase family
Tissue Specificity
Expressed in roots, stems, leaves, seedlings, inflorescence tips, and siliques.
Database Links

KEGG: ath:AT1G53500

STRING: 3702.AT1G53500.1

UniGene: At.20215

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