crb2 Antibody

Datasheet

Code	CSB-PA308549XA01SXV
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) crb2 Polyclonal antibody

Uniprot No.

P87074

Target Names

crb2

Alternative Names

crb2 antibody; rhp9 antibody; SPBC342.05 antibody; DNA repair protein crb2 antibody; Checkpoint mediator protein crb2 antibody; Cut5-repeat binding protein 2 antibody; RAD9 protein homolog antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) crb2 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage. Interaction with rad4 via its phosphorylation sites in the N-terminus couples the DNA checkpoint apparatus to chromatin via interaction of its C-terminal BRCT domains with epigenetic modifications on histones H4 and H2A, respectively, in the G1/S phase of the cell cycle, and facilitates recruitment of the checkpoint kinase chk1.

Gene References into Functions

Data show that polar interactions of Crb2 serine-548 and lysine-619 with the phosphate group of phospho-H2A (gamma-H2A) are critical for Crb2 IRIF formation and checkpoint function. PMID: 20679485
Findings argue that binding of the Crb2 BRCT repeats to pH2AX is critical for checkpoint activity and provide new insight into the mechanisms of chromatin-mediated genome stability. PMID: 20679488
When the Crb2 mutants experience damaged replication forks upon inhibition of the religation activity of topoisomerase I, the Chk1 DNA damage pathway induces sustained activation of the spindle checkpoint PMID: 16107732
Full Crb2 activity requires phosphorylation of threonine-215 by Cdc2. PMID: 16314498
Histone modifications cooperate in the same Crb2 recruitment pathway, which also requires the Tudor and BRCT motifs in Crb2. PMID: 16778077
Structural and functional analysis of the crb2 domain reveals distinct roles in checkpoint signaling and DNA damage repair. PMID: 18676809

Hide All

Subcellular Location

Nucleus.

Database Links

KEGG: spo:SPBC342.05

STRING: 4896.SPBC342.05.1

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Hippo signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

crb2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background