Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RIC1 Polyclonal antibody

Uniprot No.

F4IVV0

Target Names

RIC1

Alternative Names

RIC1 antibody; At2g33460 antibody; F4P9.23 antibody; CRIB domain-containing protein RIC1 antibody; ROP-interactive CRIB motif-containing protein 1 antibody; Target of ROP protein RIC1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RIC1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Functions as downstream effector of Rho-related GTP binding proteins of the 'Rho of Plants' (ROPs) family. Participates in the propagation of ROP GTPase signals in specific cellular responses. Required for cortical microtubule organization. Promotes microtubule bundling and formation of well-ordered microtubule arrays in the neck region of pavement cells. This restricts cell lateral expansion to generate the narrow neck morphology of pavement cells. Its function is inhibited when it interacts with activated ARAC4/ROP2. Represses ARAC4/ROP2 activation and antagonizes the RIC4-actin pathway that promotes the assembly of cortical actin microfilaments. Acts as downstream effector of ARAC3/ROP6 which functions in a signaling pathway that negatively regulates clathrin-mediated endocytosis and internalization of PIN1 and PIN2. Required for the asymmetric auxin distribution during root gravitropism and vascular patterning. Positively regulates auxin responses, but negatively regulates ABA responses during lateral root development and primary root elongation.

Gene References into Functions

ROP2-RIC1 pathway fine-tunes microtubule dynamics in response to salt stress in Arabidopsis.ROP2 participates in salt tolerance through suppressing RIC1. PMID: 28070891
Data indicate that the cellulase-induced inhibition of cell interdigitation was not observed in a RIC1 knockout mutant. PMID: 28011873
Data propose that RIC1 regulates F-actin dynamics at the apical PM as well as the cytosol by severing F-actin and capping the barbed ends in the cytoplasm, establishing a novel mechanism that underlies the regulation of pollen tube growth. PMID: 25804540
RIC1 knockout suppresses the effect of auxin on lateral root formation and primary root elongation. PMID: 23078108
The ROP6-RIC1 pathway links cytoplasmic auxin signaling to the regulation of cortical microtubule organization and cell elongation PMID: 23394835
SPIKE1 (SPK1) mutations induced PIN2 internalization not suppressed by auxin, as did the loss-of-function mutations for Rho-like GTPase from Plants 6-GTPase or its effector RIC1. SPK1 was required for auxin induction of ROP6 activation. PMID: 22683260
ROP6 and its downstream effector RIC1 regulate clathrin association with plasma membrane for clathrin-mediated endocytosis, as well as for its feedback regulation by auxin. Genetic analyses show ROP6/RIC1 acts downstream of ABP1 to regulate endocytosis. PMID: 22683261

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Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm.

Tissue Specificity

Expressed in columella cells from the root tip and epidermal cells at the base of lateral roots, leaves, stems, flowers, anthers, pollen and siliques.

Database Links

KEGG: ath:AT2G33460

STRING: 3702.AT2G33460.1

UniGene: At.37923

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RIC1 Antibody

Product Details

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Target Background