Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans ric-8 Polyclonal antibody

Uniprot No.

Q9GSX9

Target Names

ric-8

Alternative Names

ric-8 antibody; Y69A2AR.2 antibody; Synembryn antibody; Resistance to inhibitors of cholinesterase protein 8 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans ric-8 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins independently of G-protein coupled receptors. Acts by exchanging bound GDP for free GTP. Able to facilitate synaptic transmission in the nervous system probably by activating G(q)-alpha (egl-30). Also able to activate the G(s)-alpha in synaptic signaling network. Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i)-alpha protein goa-1 and gpa-16 independently of G-protein coupled receptors. While it acts as a GEF for goa-1, it has no GEF activity toward gpa-16. In addition to its GEF activity, it is required for cortical subcellular localization of G-alpha proteins such as gpa-16. Also required for the interaction of goa-1 and gpr-1/2, suggesting that it may act by generating G-alpha proteins free from G-beta-gamma subunits, enabling gpr-1/2 to mediate asymmetric cell division.

Gene References into Functions

Genetic epistasis experiments show that activator of G protein signaling (AGS-3) and guanine nucleotide exchange factor RIC-8 act during food deprivation in a mutually dependent fashion to activate G protein Galpha(o). PMID: 21832186
ric-8 (synembryn) paralysis can be rescued by neuronal G alpha(s) pathway activation. PMID: 15489510
Analysis of a ric-8 mutant suggests that it is required to maintain both the G alpha(q) vesicle priming pathway and the neuronal G alpha(s) pathway in a functional state. PMID: 15489511
RIC-8 directly modulates Galpha activity and that Galpha-GTP is the signaling molecule regulating spindle positioning in the early embryo PMID: 15498497

Subcellular Location

Cytoplasm, cell cortex. Note=Localizes to the cell cortex. Excluded from interphase nuclei and becomes localized around what appeared to be kinetochore microtubules at the onset of mitosis, becoming more diffuse during anaphase.

Protein Families

Synembryn family

Tissue Specificity

Present throughout the nervous system in juveniles and adults (at protein level).

Database Links

KEGG: cel:CELE_Y69A2AR.2

STRING: 6239.Y69A2AR.2a

UniGene: Cel.19475

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ric-8 Antibody

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