ric8a Antibody

Code CSB-PA895234XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) ric8a Polyclonal antibody
Uniprot No.
Target Names
ric8a
Alternative Names
ric8a antibody; l(1)G0397 antibody; Ric8 antibody; CG15797 antibody; Synembryn antibody; Protein Ric-8 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) ric8a protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins independently of G-protein coupled receptors. Acts by exchanging bound GDP for free GTP. Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i) alpha protein independently of G-protein coupled receptors. In addition to its GEF activity, it plays an essential role in cortical subcellular localization of heterotrimeric G proteins, suggesting it acts as a facilitator of G-alpha function through control of its membrane targeting and/or assembling of associated components rather than a GEF. Also required during gastrulation and sensory organ precursors (SOP) formation.
Gene References into Functions
  1. the small molecule stabilizes a mobile C-terminal helix inside a hydrophobic crevice of NCS-1 to impede Ric8a interaction. PMID: 28119500
  2. Data indicate that Ric-8 binds to Galpha12/13 subunit, Concertina (Cta) through an interface of conserved residues. PMID: 24006487
  3. Ric-8, which belongs to a family of guanine nucleotide-exchange factors for Galphai, regulates cortical localization of the subunits Galphai and Gbeta13F. PMID: 16228010
  4. both receptor-dependent and receptor-independent G-protein functions are executed at the plasma membrane and require the Ric-8 protein. PMID: 16228011
  5. Ric-8 homologue is required for asymmetric division of both NBs and pl cells.Ric-8 is necessary for membrane targeting of Galphai, Pins and Gbeta13F PMID: 16228012
  6. New studies show that targeting of G-protein subunits to membrane requires Ric-8, pointing to possible novel roles for this protein in both receptor-dependent and independent pathways. PMID: 16385732

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Subcellular Location
Cytoplasm, cell cortex. Note=Localizes to the cell cortex.
Protein Families
Synembryn family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: dme:Dmel_CG15797

STRING: 7227.FBpp0071252

UniGene: Dm.6684

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