RID2 Antibody

Datasheet

Code	CSB-PA196247XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RID2 Polyclonal antibody

Uniprot No.

Q9LVD0

Target Names

RID2

Alternative Names

RID2 antibody; At5g57280 antibody; MJB24.9 antibody; 18S rRNA antibody; guanine-N(7))-methyltransferase RID2 antibody; EC 2.1.1.309 antibody; Protein ROOT INITIATION DEFECTIVE 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RID2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Essential protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Involved in the pre-rRNA processing steps in the nucleolus leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Supports cell proliferation. Required for the initiation of lateral root primordia formation and for the root apical meristem (RAM) organization as well as for leaves development. During callus formation from hypocotyl and root explants, required for the initial stage of reactivation of cell proliferation in the hypocotyl stele. Involved in leaf polarity establishment by functioning cooperatively with AS2 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures.

Gene References into Functions

RID2 contributes to the nucleolar activity for pre-rRNA processing, probably through some methylation reaction. PMID: 21401745

Subcellular Location

Nucleus. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm. Nucleus, nucleolus.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, BUD23/WBSCR22 family

Tissue Specificity

Expressed in seedlings, roots and flowers.

Database Links

KEGG: ath:AT5G57280

STRING: 3702.AT5G57280.1

UniGene: At.28542

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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