rig Antibody

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Code	CSB-PA801177XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) rig Polyclonal antibody

Uniprot No.

Q86BY9

Target Names

rig

Alternative Names

rig antibody; CG30149 antibody; Protein rigor mortis antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) rig protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Nuclear receptor cofactor for the ecdysone-regulated processes of molting and puparium formation. Acts downstream from ecdysone biosynthesis and release to control the expression of specific ecdysone-regulated genes such as Eip74EF (E74).

Gene References into Functions

Dynamic intracellular redistribution of Rig protein suggests that it may act to refine spatial and temporal responses to ecdysone during development. PMID: 14645129

Subcellular Location

Nucleus. Cytoplasm. Note=During second and third instar larval development, it is localized primarily to the cytoplasm of cells in the brain and imaginal disks (tissues that are fated to form specific parts of the adult fly during metamorphosis). Also localized to the cytoplasm of larval salivary gland cells during the second and early third instar stages. It however, begins to shift into the nucleus of these cells in the mid-third instar maintaining this localization through the end of larval development. At puparium formation, it shuttles out of the nucleus of salivary gland cells to become more abundant in the cytoplasm. A similar dynamic movement occurs in cells of the larval midgut during third instar development. Component of U bodies.

Tissue Specificity

Expressed in the brain and salivary glands of early and late second instar larvae. Expressed in nurse cells and oocytes.

Database Links

KEGG: dme:Dmel_CG30149

STRING: 7227.FBpp0085564

UniGene: Dm.29482

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rig Antibody

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