RIM15 Antibody

Code CSB-PA337446XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIM15 Polyclonal antibody
Uniprot No.
Target Names
RIM15
Alternative Names
RIM15 antibody; TAK1 antibody; YFL033C antibody; Serine/threonine-protein kinase RIM15 antibody; EC 2.7.11.1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIM15 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein kinase that positively regulates proper entry into stationary phase of cells under nutrient starvation conditions. Involved in glycogen and trehalose accumulation, derepression of stress-induced genes, induction of thermotolerance and starvation resistance, and proper G1 cell cycle arrest. Also involved in the activation of a meiotic genes activation pathway. Phosphorylates IGO1 and IGO2, both involved in the TORC1 control of gene expression and chronological life span.
Gene References into Functions
  1. Rim15 activity increases after cells switch from fermentation to respiration, where Igo1,2 contribute to chromosome maintenance. Interestingly Cln3-Cdk1 also inhibits Rim15 activity, which enables homeostatic control of Whi5 phosphorylation and cell cycle entry. PMID: 28600888
  2. Rim15, a Greatwall-family protein kinase in Saccharomyces cerevisiae, is required for cellular nutrient responses, such as the entry into quiescence and the induction of meiosis and sporulation. (Review) PMID: 28485209
  3. ur data support the hypothesis that metabolic reprogramming to accumulate energy stores and the activation of anti-oxidant defence systems are coordinated by Yak1, Rim15 and Mck1 kinases to ensure quiescence entry and lifespan extension in yeast. PMID: 27923067
  4. Validity of promoter engineering of the RIM15 gene that governs inhibitory control of alcoholic fermentation. PMID: 27633130
  5. G0/G1 transition, via Rim15 and CLN3, might govern the fermentation rate of bottom-fermenting brewer's yeast in high-gravity wort. PMID: 27212268
  6. Rim15 and Sch9 kinases are involved in induction of autophagic degradation of ribosomes in budding yeast PMID: 27659307
  7. A null RIM15 variant confers a greater fermentation rate through the utilization of available glucose instead of its storage. However, this variant has a detrimental effect on fungicide resistance since complex sugars are not synthesized and transported into the membrane. PMID: 26945894
  8. Mpk1 and the greatwall kinase (RIM15) pathway serve TORC1 to coordinate the phosphorylation status of Sic1 and consequently G1-S cell cycle progression with nutrient availability. PMID: 26356805
  9. We propose that the Rim15-Endosulfine( Igo1 and Igo2 )-PP2ACdc55 signalling module triggers entry into quiescence and gametogenesis by regulating dephosphorylation of distinct substrates PMID: 24968058
  10. failure of rim15 cells to adapt to calorie restriction by entering robust post-mitotic state resembles cancer physiology and shows retentostat cultivation of yeast strains can provide relevant models for healthy post-mitotic and transformed human cells PMID: 24487068
  11. We conclude that Greatwall, endosulfines, and PP2A are part of a regulatory module that has been conserved during evolution irrespective of PP2A function in the control of mitosis PMID: 23861665
  12. Rim15 can phosphorylate Msn2, but not Gis1, in vitro, implying different mechanisms for the activation of these transcription factors. PMID: 24140345
  13. Rim15 is required for the biological activity of triclabendazole in the increased resistance to stress. PMID: 23667708
  14. We present data supporting that the PHO pathway functions in parallel to the fermentable growth medium- or Sch9-controlled pathway and that both pathways may share the protein kinase Rim15 in Saccharomyces PMID: 15926040
  15. Rim15 plays a key role in G0 entry through its ability to integrate signaling from the PKA, TORC1, and Pho80-Pho85 pathways PMID: 16308562
  16. These results indicate that the Ras/cAMP/PKA/Rim15/Msn2/4 and the Tor/Sch9/Rim15/Gis1 pathways are major mediators of the calorie restriction-dependent stress resistance and life span extension. PMID: 18225956

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Database Links

KEGG: sce:YFL033C

STRING: 4932.YFL033C

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