RIPK Antibody

Datasheet

Code	CSB-PA246787XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RIPK Polyclonal antibody

Uniprot No.

Q9ZUF4

Target Names

RIPK

Alternative Names

RIPK antibody; PBL14 antibody; PIX8 antibody; At2g05940 antibody; Serine/threonine-protein kinase RIPK antibody; EC 2.7.11.1 antibody; ACIK1A/PBL14 antibody; ACRE protein ortholog 264A antibody; AtACRE264a antibody; PBS1-like protein 14 antibody; RPM1-induced protein kinase antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RIPK protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine-protein kinase involved in disease resistance. During Pseudomonas syringae infection, and in response to the bacterial effectors AvrB and AvrRpm1, RIPK phosphorylates the host target RIN4, which subsequently activates RPM1-dependent effector-triggered immunity (ETI). Seems to act as negative regulator of plant basal defense responses and may play a role in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). Required for the bacterial XopAC/AvrAC effector-triggered immunity against Xanthomonas campestris.

Gene References into Functions

RALF1 triggers the phosphorylation of both FER and RIPK in a mutually dependent manner PMID: 27930296
AvrB and AvrPphB target a conserved defense signaling pathway in Arabidopsis and soybean that includes RIPK and RIN4. PMID: 25625821
Xanthomonas campestris pathovar campestris type III effector AvrAC enhances virulence and inhibits plant immunity by specifically targeting Arabidopsis BIK1 and RIPK, two receptor-like cytoplasmic kinases known to mediate immune signalling.[RIPK] PMID: 22504181
xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK. PMID: 23951354
RIPK (At2g05940) phosphorylates the host target RIN4, leading to the activation of RPM1 during Pseudomonas syringae infection. PMID: 21320696

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Subcellular Location

Cell membrane.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: ath:AT2G05940

STRING: 3702.AT2G05940.1

UniGene: At.23011

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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