RPL10 Antibody

Code CSB-PA020106XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPL10 Polyclonal antibody
Uniprot No.
Target Names
RPL10
Alternative Names
RPL10 antibody; GRC5 antibody; QSR1 antibody; YLR075W antibody; 60S ribosomal protein L10 antibody; L9 antibody; Large ribosomal subunit protein uL16 antibody; Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPL10 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.
Gene References into Functions
  1. The T-ALL related rpl10-R98S mutation blocks 60S biogenesis by a mechanism distinct from other rpl10 mutations. The rpl10-R98S mutant is suppressed by mutations in NMD3 and in TIF6. PMID: 28715419
  2. correct loading of Rpl10p into 60S subunits is required for the release of Nmd3p from subunits by Lsg1p PMID: 15660131
  3. mutant Rpl10 proteins were engineered to be unable to bind to the ribosome accumulated in the nucleus PMID: 17761675
Subcellular Location
Cytoplasm.
Protein Families
Universal ribosomal protein uL16 family
Database Links

KEGG: sce:YLR075W

STRING: 4932.YLR075W

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