Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPL3 Polyclonal antibody

Uniprot No.

P14126

Target Names

RPL3

Alternative Names

RPL3 antibody; MAK8 antibody; TCM1 antibody; YOR063W antibody; YOR29-14 antibody; 60S ribosomal protein L3 antibody; Large ribosomal subunit protein uL3 antibody; Maintenance of killer protein 8 antibody; RP1 antibody; Trichodermin resistance protein antibody; YL1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPL3 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL3 plays a role in coordinating processes of accommodating the aminoacyl-tRNA in the PTC.

Gene References into Functions

Rpl3 methylation at H243 is playing a significant role in translation elongation. PMID: 26826131
We conclude that L3 plays an important role in the function of eIF5B in stimulating 3' end processing of 18S rRNA in the context of 80S ribosomes that have not yet engaged in translation. PMID: 24603549
3-methylhistidine modification of Rpl3 is dependent upon the YIL110W methyltransferase. PMID: 20864530
To understand how ribosomal proteins may influence ribosome function, the large-scale mutational analysis of ribosomal protein L3 was performed. PMID: 16314511
A mutant gene responsible for curvularol resistance was identified as an allele of the RPL3 gene encoding the ribosomal protein L3. PMID: 17031058
Upon depletion of Rpl3p, early assembly of 60S ribosomal subunits is aborted and subsequent steps during their maturation and export prevented. PMID: 17569673

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Universal ribosomal protein uL3 family

Database Links

KEGG: sce:YOR063W

STRING: 4932.YOR063W

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA020224XA01SVG
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

RPL3 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background