RLK5 Antibody

Code CSB-PA342515XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RLK5 Polyclonal antibody
Uniprot No.
Target Names
RLK5
Alternative Names
RLK5 antibody; HAE antibody; At4g28490 antibody; F21O9.180 antibody; Receptor-like protein kinase 5 antibody; EC 2.7.10.1 antibody; EC 2.7.11.1 antibody; Protein HAESA antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RLK5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.
Gene References into Functions
  1. HAE role in the pectin degradation and resistance to Pseudomonas syringae in Arabidopsis leaves. PMID: 27618493
  2. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. PMID: 27058169
  3. The study reveals the signaling activation mechanism in floral organ abscission by IDA-induced HAE/HSL2-SERK complex formation accompanied by transphosphorylation. PMID: 26854226
  4. Results provide evidence for a conserved phosphorylation site on a known activation domain additionally to another putative activation segment phosphorylation site that potentially modulates HAE activity and substrate specificity. PMID: 26784444
  5. HAE HSL2-dependent and HAE HSL2-independent changes in genes expression are required for abscission PMID: 23327667
  6. Floral organ abscission peptide IDA and its HAE/HSL2 receptors control cell separation during lateral root emergence. PMID: 23479623
  7. Data show that through the receptor-like kinases HAESA and HAESA-LIKE2, IDA and the IDL proteins constitute a family of putative ligands to regulate different events during plant development. PMID: 18660431

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed in roots and rosettes. Expressed at the base of petioles and pedicels, and in the abscission zones of the floral organs.
Database Links

KEGG: ath:AT4G28490

STRING: 3702.AT4G28490.1

UniGene: At.19985

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