rlmI Antibody

Code CSB-PA305129XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rlmI Polyclonal antibody
Uniprot No.
Target Names
rlmI
Alternative Names
rlmI antibody; yccW antibody; b0967 antibody; JW5898Ribosomal RNA large subunit methyltransferase I antibody; EC 2.1.1.191 antibody; 23S rRNA m5C1962 methyltransferase antibody; rRNA antibody; cytosine-C(5)-)-methyltransferase RlmI antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rlmI protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits.
Gene References into Functions
  1. Results report the identity of a third E. coli m(5)C methyltransferase, YccW. PMID: 18786544
  2. Results report the crystal structure of the 23S rRNA:m5C methyltransferase RlmI refined at 2 A to a final R-factor of 0.194 (R(free)=0.242). PMID: 18789337
Subcellular Location
Cytoplasm.
Protein Families
Methyltransferase superfamily, RlmI family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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