rlmL Antibody

Datasheet

Code	CSB-PA305128XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rlmL Polyclonal antibody

Uniprot No.

P75864

Target Names

rlmL

Alternative Names

rlmL antibody; rlmK antibody; rlmKL antibody; ycbY antibody; b0948 antibody; JW0931 antibody; Ribosomal RNA large subunit methyltransferase K/L [Includes: 23S rRNA m2G2445 methyltransferase antibody; EC 2.1.1.173 antibody; rRNA antibody; guanine-N(2)-)-methyltransferase RlmL); 23S rRNA m7G2069 methyltransferase antibody; EC 2.1.1.264 antibody; rRNA antibody; guanine-N(7)-)-methyltransferase RlmK)] antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rlmL protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits.

Gene References into Functions

E. coli YcbY is a bifunctional rRNA methyltransferase catalyzing the m2G2445 and m7G2069 modifications located on opposite sides of helix 74. PMID: 22362734
RlmKL is a novel type of fused methyltransferase bearing dual active sites responsible for different methyl modifications at distinct positions. PMID: 22210896
final crystals of RlmL diffracted to 2.2 A resolution PMID: 21045301
Results describe the identification of a gene, ycbY, as encoding an enzyme responsible for methylation of 23 S rRNA G2445. PMID: 17010378

Subcellular Location

Cytoplasm.

Protein Families

Methyltransferase superfamily, RlmKL family

Database Links

KEGG: ecj:JW0931

STRING: 316385.ECDH10B_1018

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rlmL Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background