Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rlmN Polyclonal antibody

Uniprot No.

P36979

Target Names

rlmN

Alternative Names

rlmN antibody; yfgB antibody; b2517 antibody; JW2501 antibody; Dual-specificity RNA methyltransferase RlmN antibody; EC 2.1.1.192 antibody; 23S rRNA antibody; adenine(2503)-C(2))-methyltransferase antibody; 23S rRNA m2A2503 methyltransferase antibody; Ribosomal RNA large subunit methyltransferase N antibody; tRNA antibody; adenine(37)-C(2))-methyltransferase antibody; tRNA m2A37 methyltransferase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rlmN protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity. Unmodified tRNA is not a suitable substrate for RlmN, which suggests that RlmN works in a late step during tRNA maturation.

Gene References into Functions

this study reports the x-ray structure of a key intermediate in the RlmN reaction, in which a Cys(118)-->Ala variant of the protein is cross-linked to a tRNA(Glu)substrate through the terminal methylene carbon of a formerly methylcysteinyl residue and C2 of adenosine 37. PMID: 27081063
RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy. PMID: 22891362
Reconstitution of the [4Fe-4S] cluster on wild-type apo RlmN followed by addition of S-adenosylmethionine results in rapid production of S-adenosylhomocysteine and the mCys residue. PMID: 21916495
structure of RlmN and RlmN with SAM show single molecule of SAM coordinates [4Fe-4S] cluster; residue Cys(355) is S-methylated and located proximal to SAM methyl group, suggesting SAM that is involved in the initial methyl transfer binds at the same site PMID: 21527678
SAM enzymes identified that methylate C atoms not inherently nucleophilic via intermediacy of 5'-deoxyadenosyl 5'-radical; determined reaction mechanism targeting sp(2)-hybridized carbons at position 2 and 8 of adenosine in 23S RRNA catalyzed by RlmN, Cfr PMID: 21415317

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Subcellular Location

Cytoplasm.

Protein Families

Radical SAM superfamily, RlmN family

Database Links

KEGG: ecj:JW2501

STRING: 316385.ECDH10B_2683

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rlmN Antibody

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Target Background