rluF Antibody

Datasheet

Code	CSB-PA329191XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rluF Polyclonal antibody

Uniprot No.

P32684

Target Names

rluF

Alternative Names

rluF antibody; yjbC antibody; b4022 antibody; JW3982 antibody; Dual-specificity RNA pseudouridine synthase RluF antibody; EC 5.4.99.- antibody; EC 5.4.99.21 antibody; 23S rRNA pseudouridine(2604) synthase antibody; Ribosomal large subunit pseudouridine synthase F antibody; rRNA pseudouridylate synthase F antibody; rRNA-uridine isomerase F antibody; tRNA(Tyr) pseudouridine(35) synthase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rluF protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA and from uracil-35 in the anticodon of tRNA(Tyr). Can, to a small extent, also react with uracil-2605.

Gene References into Functions

Analysis of total tRNA isolated from E. coli pseudouridine synthase knock-out mutants identified RluF as the enzyme responsible for this modification. PMID: 27551044
Results describe the crystal structure of an RluF-RNA complex, and suggest that a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome. PMID: 19298824

Protein Families

Pseudouridine synthase RsuA family

Database Links

KEGG: ecj:JW3982

STRING: 316385.ECDH10B_4211

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rluF Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background