rmf Antibody

Code CSB-PA365366XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rmf Polyclonal antibody
Uniprot No.
Target Names
rmf
Alternative Names
rmf antibody; b0953 antibody; JW0936 antibody; Ribosome modulation factor antibody; RMF antibody; Hibernation factor RMF antibody; Protein E antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rmf protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
During stationary phase, converts 70S ribosomes to an immature dimeric form (90S ribosomes) which are converted to inactive 100S ribosomes (a process called ribosomal hibernation) by the hibernation promoting factor HPF. Inactivates ribosomes by covering the peptidyl transferase (PTase) center of the 23S rRNA and the entrance of peptide exit tunnel. However crystallization with T.thermophilus 70S ribosomes shows it binds near the 3'-end of the 16S rRNA near the anti-Shine-Dalgarno sequence, where it would sterically hinder translation inititation. In this crystal binding of RMF induces movement of the 30S head domain away from the rest of the ribosome, presumably so they would more easily form dimers. Also involved in protection against heat stress, but this role is not dependent on the maintenance of ribosome dimers.
Gene References into Functions
  1. Data propose that CRP regulates transcription activation of the rmf gene for formation of 100S ribosome dimers. PMID: 23475967
  2. crystal structure of Thermus thermophilus 70S ribosome in complex with stationary-phase factors RMF, HPF and YfiA; binding site of RMF overlaps with that of mRNA Shine-Dalgarno sequence, which prevents interaction between the mRNA and the 16S ribosomal RNA PMID: 22605777
  3. Ribosomes change into forms which are hard to bind with IF3 and easy to form 100S ribosomes by RMF and HPF concomitantly with transition from the log phase to the stationary phase. PMID: 19170772
  4. Data showed that FtsY depletion inhibits the translation of both SRP-dependent and independent proteins. This synthesis defect is the result of a multifaceted response that includes the upregulation of the ribosome-inactivating protein RMF. PMID: 19749044
Subcellular Location
Cytoplasm.
Protein Families
Ribosome modulation factor family
Database Links
CUSABIO guaranteed quality
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