RMI1 Antibody

Code CSB-PA824000XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RMI1 Polyclonal antibody
Uniprot No.
Target Names
RMI1
Alternative Names
RMI1 antibody; At5g63540 antibody; MLE2.17 antibody; RecQ-mediated genome instability protein 1 antibody; BLM-associated protein of 75 kDa homolog antibody; BLAP75 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RMI1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential component of the RMI complex, a complex that plays an important role in the resolution step of homologous recombination, in a process called Holliday Junction dissolution, to limit DNA crossover formation in cells. Together with TOP3A, is essential for the resolution of meiotic recombination intermediates, a step that prevents entanglement of the parental chromosomes.
Gene References into Functions
  1. The results show that the DUF1767 domain and the OB-fold domain 1 are both essential for the function of AtRMI1 in DNA cross-link repair as well as meiotic recombination, but partially dispensable for somatic homologous recombination suppression. PMID: 23956219
  2. BLAP75/Rmi1 is a key protein of the meiotic homologous recombination machinery. PMID: 19096505
  3. AtTOP3alpha and AtRMI1 are involved in the suppression of crossover recombination in somatic cells and DNA repair. PMID: 19096507
Protein Families
RMI1 family
Database Links

KEGG: ath:AT5G63540

UniGene: At.51108

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