RNE Antibody

Datasheet

Code	CSB-PA220539XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RNE Polyclonal antibody

Uniprot No.

F4IV66

Target Names

RNE

Alternative Names

RNE antibody; RNEE/G antibody; At2g04270 antibody; T23O15.10 antibody; Ribonuclease E/G-like protein antibody; chloroplastic antibody; RNase E/G-like protein antibody; EC 3.1.26.- antibody; RNase E antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RNE protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Involved in intercistronic processing of primary transcripts from chloroplast operons. The endonucleolytic activity of the enzyme depends on the number of phosphates at the 5' end, is inhibited by structured RNA, and preferentially cleaves A/U-rich sequences.

Gene References into Functions

Arabidopsis CSP41 proteins form multimeric complexes that bind and stabilize distinct plastid transcripts. PMID: 22090436
RNase E mutants have multiple defects in chloroplast RNA metabolism, but RNase E is not essential for survival. PMID: 21105931
RNase E participates in the initial endonucleolytic cleavage of the polyadenylation-stimulated RNA degradation process in the chloroplast, perhaps in collaboration with the two other chloroplast endonucleases, RNase J and CSP41. PMID: 18441049
Loss of At2g04270 function resulted in the arrest of chloroplast development, loss of autotrophic growth, and reduced plastid ribosomal, psbA and rbcL RNA levels. PMID: 18515828

Subcellular Location

Plastid, chloroplast stroma.

Protein Families

RNase E/G family

Tissue Specificity

Expressed in cotyledons, rosette and cauline leaves.

Database Links

KEGG: ath:AT2G04270

STRING: 3702.AT2G04270.5

UniGene: At.41329

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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