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RING1A Antibody

Datasheet
Code CSB-PA880778XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RING1A Polyclonal antibody
Uniprot No.
Q9FKW0
Target Names
RING1A
Alternative Names
RING1A antibody; RF434 antibody; At5g44280 antibody; K9L2.3 antibody; Putative E3 ubiquitin-protein ligase RING1a antibody; EC 2.3.2.27 antibody; Polycomb complex protein RING1a antibody; Protein RING1a antibody; AtRING1a antibody; RING-type E3 ubiquitin transferase RING1a antibody; Ring finger protein 434 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RING1A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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RING1A Proteins

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Target Background

Function
Putative E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119ub), thereby playing a central role in histone code and gene regulation.; As part of the PRC1-like complex, repress class I KNOX gene expression. PcG PRC1 complex maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility.
Gene References into Functions
  1. RING1A suppresses the expression of MAF4 and MAF5 through affecting H3K27me3 levels at these loci to regulate the floral transition PMID: 24553292
  2. AtRING1a is elevated in the Atbmi1a Atbmi1b mutant, suggesting a self-regulatory feedback mechanism. PMID: 21060339
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT5G44280

UniGene: At.30083

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