rng2 Antibody

Code CSB-PA515651XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) rng2 Polyclonal antibody
Uniprot No.
Target Names
rng2
Alternative Names
rng2 antibody; SPAC4F8.13c antibody; Ras GTPase-activating-like protein rng2 antibody; Ring assembly protein 2 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) rng2 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Required for cytokinesis. Component of the contractile F-actin ring; required for its construction following assembly of F-actin at the division site.
Gene References into Functions
  1. Data indicate several distinct functions of Rng2 at multiple stages of cytokinesis. PMID: 23615450
  2. Mid1p recruits Rng2p to cortical nodes at the cell-division site and Rng2p, in turn, recruits other components of the actomyosin ring to cortical nodes, thereby ensuring correct placement of the division site. PMID: 21376595
  3. The 2.21 angstrom resolution crystal structure of the amino-terminal 190 amino acids of Rng2, including the calponin-homology domain and 40 residues that do not share sequence similarity with known proteins. [Rng2] PMID: 15272162
  4. Findings indicate that Rng2 is involved in the generation of CR F-actin and simultaneously bundles the filaments and regulates its dynamics by counteracting the effects of Adf1. PMID: 19713940
  5. The IQGAP-related Rng2 protein is a component of the actomyosin ring and spindle pole body, and is required for actomyosin ring construction following assembly of F-actin at the division site. PMID: 9635188

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Subcellular Location
Cytoplasm, cytoskeleton. Nucleus envelope. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Note=Localized to the F-actin ring and spindle pole body during interphase and mitosis. Also found in septum.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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