RNH201 Antibody

Code CSB-PA019805XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RNH201 Polyclonal antibody
Uniprot No.
Target Names
RNH201
Alternative Names
RNH201 antibody; RNH35 antibody; YNL072W antibody; N2369 antibody; Ribonuclease H2 subunit A antibody; RNase H2 subunit A antibody; EC 3.1.26.4 antibody; RNase H(201) antibody; RNase H(35) antibody; Ribonuclease HI large subunit antibody; RNase HI large subunit antibody; Ribonuclease HI subunit A antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RNH201 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
Gene References into Functions
  1. Both R-loop removal and RER activities of RNase H2 contribute substantially to chromosome stability, and that their relative contributions may be variable across different regions of the genome. In this scenario, a prominent contribution of R-loop removal may be expected at highly transcribed regions, whereas RER may play a greater role at hotspots of ribonucleotide incorporation. PMID: 28268090
  2. An important role for RNase H2 in maintaining genome integrity by removing single ribonucleotides incorporated during DNA replication is shown. PMID: 28325498
  3. Deleting RNH201 elicits a strong cellular stress response in S. cerevisiae. PMID: 23079308
  4. study presents a complete biochemical reconstitution of the ribonucleotide excision repair (RER) pathway; RER is most efficient when the ribonucleotide is incised by RNase H2 PMID: 22864116
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
RNase HII family, Eukaryotic subfamily
Database Links

KEGG: sce:YNL072W

STRING: 4932.YNL072W

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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