rnlA Antibody

Code CSB-PA346731XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rnlA Polyclonal antibody
Uniprot No.
Target Names
rnlA
Alternative Names
rnlA antibody; std antibody; yfjN antibody; b2630 antibody; JW2611 antibody; mRNA endoribonuclease toxin LS antibody; EC 3.1.-.- antibody; RNase LS antibody; Toxin LS antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rnlA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Toxic component of a type II toxin-antitoxin (TA) system. A stable (half-life 27.6 minutes) endoribonuclease that in the absence of cognate antitoxin RnlB causes generalized RNA degradation. Degrades late enterobacteria phage T4 mRNAs, protecting the host against T4 reproduction. Activity is inhibited by cognate antitoxin RnlB and by enterobacteria phage T4 protein Dmd. Targets cyaA mRNA.
Gene References into Functions
  1. Results suggest that RnlA functions as a toxin with mRNA endoribonuclease activity and is inhibited after binding to T4 antitoxin Dmd protein through its Dmd-binding domain. PMID: 24112600
  2. RNase LS cleaves soc RNA in a manner dependent on translation termination. PMID: 16020788
  3. Fractionation of the RNase LS activity in a cell extract showed that it sedimented through a sucrose density gradient as a 1000-kDa complex that included RnlA. Pull-down experiments revealed more than 10 proteins associated with His-tagged RnlA. PMID: 17895580
  4. RNase LS regulates Crp-cAMP concentration by degrading the cyaA transcripts. PMID: 19019153
Subcellular Location
Cytoplasm.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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