Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rnr Polyclonal antibody

Uniprot No.

P21499

Target Names

rnr

Alternative Names

rnr antibody; vacB antibody; yjeC antibody; b4179 antibody; JW5741 antibody; Ribonuclease R antibody; RNase R antibody; EC 3.1.13.1 antibody; Protein VacB antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rnr protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (rRNAs, tRNAs and SsrA/tmRNA). In stationary phase, involved in the post-transcriptional regulation of ompA mRNA stability. Shortens RNA processively to di- and trinucleotides. In vitro, exhibits helicase activity, which is independent of its RNase activity. RNases 2 and R (rnb and this entry) contribute to rRNA degradation during starvation, while RNase R and PNPase (this entry and pnp) are the major contributors to quality control of rRNA during steady state growth. Required for the expression of virulence genes in enteroinvasive strains of E.coli.

Gene References into Functions

Data indicate that RNase R is a proficient enzyme, capable of concurrently binding, unwinding and degrading structured RNA in a highly processive manner during RNA decay. PMID: 29036353
These findings indicate that the intrinsic helicase activity of RNase R is required for its proper functioning in vivo and for effective RNA metabolism PMID: 27022019
The frequency downshifts of the ring and CO bands are consistent with charge transfer from YO. to W or another residue of RNR. PMID: 26627888
These findings indicate that the helicase activity plays an essential role in the catalytic efficiency of RNase R. PMID: 25931119
This study leads the authors to conclude that RNase R can interact with ribosomal proteins and that this interaction may be a result of this enzyme involvement in the ribosome quality control. PMID: 24517631
RNase R binding to ribosomes is dependent on transfer-messenger RNA (tmRNA)-SmpB, nonstop mRNA, and the modified form of ribosomal protein S12. PMID: 24133211
We show that RNR101 is stabilized in the presence of rifampicin at 42C. PMID: 21527473
analysis of growth stage-dependent modification of RNase R PMID: 21981926
The results presented here show that in fact the RNB domain from RNase R is the one responsible for the degradation of double-stranded substrates. PMID: 21465561
The authors carried out a domain analysis of RNase R and showed that this protein has two distinct activities, RNase and helicase, which are independent of each other and are due to different domains. PMID: 20023028
Data demonstrate that RNase R, which is widespread in prokaryotes and eukaryotes, is an important participant in mRNA decay. PMID: 15664199
RNAse R is dramatically increased under multiple stress conditions, which suggests extensive remodeling of structured RNA in response to the altered environment PMID: 16135521
induced in stationary phase cells and is involved in the post-transcriptional regulation of ompA mRNA. PMID: 16556233
Using a variety of specifically designed substrates, a model shows how RNase R interacts with its substrates and degrades RNA. PMID: 16893880

Hide All

Subcellular Location

Cytoplasm.

Protein Families

RNR ribonuclease family, RNase R subfamily

Database Links

KEGG: ecj:JW5741

STRING: 316385.ECDH10B_4374

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rnr Antibody

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