ROPGEF4 Antibody

Code CSB-PA174495XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ROPGEF4 Polyclonal antibody
Uniprot No.
Target Names
ROPGEF4
Alternative Names
ROPGEF4 antibody; At2g45890 antibody; F4I18.13 antibody; Rop guanine nucleotide exchange factor 4 antibody; AtRopGEF4 antibody; Rho of plants guanine nucleotide exchange factor 4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ROPGEF4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. In association with ROPGEF1, acts as specific regulator of ARAC10/ROP11 function in ABA-mediated stomatal closure.
Gene References into Functions
  1. RopGEF4 and RopGEF10 are genetic components in FER-mediated, developmentally (but not environmentally) regulated, root hair growth. PMID: 23915272
  2. ROPGEF1 and ROPGEF4 are specific regulators of ROP11 function in ABA-mediated stomatal closure PMID: 22500990
Subcellular Location
Cytoplasm. Cell membrane. Note=Interacts with ARAC10/ROP11 on plasma membrane in guard cells.
Tissue Specificity
Expressed in root vascular tissue and trichoblast cell files. Expressed in root metaxylem cell files. Expressed in guard cells of cotyledons, rosette leaves, sepals, petal, stigmas and siliques. Expressed in root metaxylem cell files.
Database Links

KEGG: ath:AT2G45890

STRING: 3702.AT2G45890.1

UniGene: At.36575

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