ROK1 Antibody

Code CSB-PA338325XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ROK1 Polyclonal antibody
Uniprot No.
Target Names
ROK1
Alternative Names
ROK1 antibody; YGL171W antibody; G1651 antibody; ATP-dependent RNA helicase ROK1 antibody; EC 3.6.4.13 antibody; Rescuer of KEM1 protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ROK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity.
Gene References into Functions
  1. Data show that ATP-bound DEAD-box protein Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 protein binding to 40S ribosomes. PMID: 27280440
  2. Rrp5 provides specificity to the otherwise nonspecific biochemical activities of the Rok1 DEAD-domain. PMID: 23630256
  3. Immunoprecipitation assays demonstrate the association of Rok1p with 35S pre-rRNA in pre-ribosomes. PMID: 15523097
  4. Rok1 was required specifically for the release of snR30. PMID: 18833290
Subcellular Location
Nucleus, nucleolus.
Protein Families
DEAD box helicase family, DDX52/ROK1 subfamily
Database Links

KEGG: sce:YGL171W

STRING: 4932.YGL171W

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